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KMID : 0380020130280040225
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Korean Journal of Biotechnology and Bioengineering
2013 Volume.28 No. 4 p.225 ~ p.229
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Carbon Dioxide Sequestration of Enzyme Covalently Immobilized on Porous Membrane
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Park Jin-Won
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Abstract
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Bovine Carbonic anhydrase (BCA) was immobilized on a submicro-porous membrane through covalent immobilization. The immobilization was conducted on the porous membrane surface with the treatment of polyethyleneimine, glutaraldehyde, and the anhydrase, in sequence. The immobilization was confirmed using X-ray photon spectrometer. The pH values of carbon-dioxide saturated solution with buffer were monitored with respect to time to calculate the catalytic activities of hydration of carbon-dioxide for free and immobilized CA. The catalytic rate constant values for free CA, immobilized CA on polystyrene nanoparticles, and immobilized CA on a porous cellulose acetate membrane were 0.79, 0.67, and 0.56 s-1, respectively. Reusability was studied up to 10 cycles of CO2 sequestration. The activity for the CA immobilized on the membrane was kept to 95% after 10 cycles, and comparable to the CA on the nanoparticles. The stabilities for heat and storage were also investigated for the three cases. The results suggested that the CA immobilized the membrane had the least loss rate of the activity compared to the others. From this study, the porous membrane was feasible as a carrier for the CA immobilization in hydration and sequestration of carbon-dioxide.
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KEYWORD
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Carbon-dioxide, Carbonic anhydrase immobilization, Porous membrane, Sequestration
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